MetroFlow: Corporate Member Launch Seminar
Propel Labs Bigfoot Cell Sorter
12:00PM – 12:15 PM - Introduction to Bigfoot
Speaker: Angie Goldfain, Scientific Director, Propel Labs
12:15PM – 12:45 PM - Bigfoot: Need for Speed - Zero to 384 in 10 seconds
Speakers: Nathan Trujillo, Ph.D., Application Scientist, Propel Labs
Michael Kissner, Director of Operations,
Columbia Stem Cell Initiative Flow Cytometry
Columbia University Irving Medical Center
12:45 – 1:00 PM - Question and Answer Session
In many plate sorting applications, mass screening has been pushing the limits of cell sorters higher than their practical capability with tradeoffs of speed vs accuracy. Accurate deposition, particularly into 384-well PCR plates, is critical for the success of many studies. Furthermore, throughput has been hampered by the need for time consuming recalibration between plates. With the development of Propel Labs’ Bigfoot cell sorter, the introduction of high speed plate mechanics, coupled with innovative algorithms in firmware, has increased accuracy to > 99% while cutting the expected plate sort time by an order of magnitude over the course of multiple plate sorts.
Bigfoot was tested with 96 and 384-well plates using the horseradish peroxidase (HRP) technique (Rodrigues & Monard, 2016) which allows the detection of single to multiple drops sorted into microtiter plates. We will review the Bigfoot Cell Sorter’s cutting-edge plate sorting features and our implementation of the HRP technique to demonstrate the instrument’s unparalleled performance.
You can find more information about the Bigfoot Cell Sorter at Propel Lab’s website: